cDNA synthesis from mRNA | How to setup the reaction | Calculation & PCR Conditions | Full Details ! cdna technology

by adminhuahinsunvilla3sd



Hello friends. This is Ashutosh K. Yadav, welcome to my you tube channel AkyBiotech. This video is about synthesis of cDNA from mRNA. You can see full details like how to put the PCR reaction and what is the PCR cycle conditions and how much RNA you have to take for synthesis of cDNA ?.

Full Video of RNA Isolation from Plant link:–
Part 1:

Real time PCR (qRT-PCR) full details video link:

Analysis of real time PCR data using delta delta CT methods (∆∆ct Method) links:

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cDNA synthesis from mRNA | How to setup the reaction | Calculation & PCR Conditions | Full Details !

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cDNA synthesis from mRNA | How to setup the reaction | Calculation & PCR Conditions | Full Details !
cdna technology
You can see a lot of useful information here: See more here
You can see a lot of useful information here: See more here

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3 comments

Swathi Reddy 11/03/2022 - 3:36 Chiều

Please upload c dna and standardization of primers using normal pcr thank u.

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Amar Kant 11/03/2022 - 3:36 Chiều

Found it helpful…. very nicely explained

Reply
Ashok Kumar 11/03/2022 - 3:36 Chiều

Sir ctab EDTA nacl 10x buffer kse bnta h

Reply

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